The Narishige PC-10 has been discontinued, replaced by the more advanced PC-100 model. The PC-100 offers similar but improved functionality and is a compatible, direct replacement. This succession is a common path in laboratory technology, but it creates a significant hurdle for existing PC-10 owners: the decreasing availability of official support and original documentation.
Before first-time use, wipe the vertical guide shaft with a lint-free cloth moistened with a small amount of light machine oil or alcohol to ensure friction-free travel.
Research communities can be excellent sources of practical knowledge. For example, the Alliance of Genome Resources forum has a discussion where a user describes their settings: "We use the PC-10 capillary puller (step 1 mode, 59 C) with the Narishige GD-1 capillaries. We center the capillaries to get two needles out of each". While not a full manual, these notes offer empirical calibration data. Dedicated forums can uncover real-world notes and tips from other users. narishige pc10 manual new
Two-stage pulling (automated change in heating/tension mid-process). No. 1 Heater: Used to manually test and set the heating value. Start Switch: Initiates the pulling sequence. ALA Scientific 2. Adjusting the Pull Characteristics
The PC-10 has several mechanical components that wear out: the heater base, the solenoid plunger, the micro-switch, and the braking mechanism. A new manual should provide an exploded view with (e.g., for the heating filament or O-rings). The Narishige PC-10 has been discontinued, replaced by
: Because it is a vertical puller, the upper and lower pipettes often have slightly different shapes due to residual heat affecting the upper portion. Key Manual & Operational Highlights
: Press the START button to begin the automated pull. Protocol Tips for Common Needs Desired Pipette Type Suggested Settings (Reference Only) Long, Thin Injection Needles High heating value + One-stage pulling Firm Patch-Clamp Electrodes Low heating value + Two-stage pulling Flexible, Sharp Tips Use all available weights to increase pull speed Gradual, Parallel Taper Use fewer weights (e.g., 2 Type Light) Maintenance and Troubleshooting NARISHIGE WEB NEWS Setting Values in the PC-10 Puller - NARISHIGE WEB NEWS Before first-time use, wipe the vertical guide shaft
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Tighten the upper clamp knob to secure the top of the glass tube.
Narishige PC-10 Glass Puller: Comprehensive Operational Manual and Setup Guide